GRP1 (K400) polyclonal antibody
Product Specifications
Background
GRP1 (general receptor for phosphoinositides-1) contains a Pleckstrin homology (PH) domain as well as a Sec7 domain. The PH domain has high binding affinity for phosphatidylinositol 3,4,5-trisphosphate Ptdlns (3,4,5) P3), while the Sec7 homology domain is responsible for catalyzing guanine nucleotide exchange of ADP-ribosylation factor (ARF) proteins. GRP1 co-localizes with ARF6 and catalyzes GTP/GDP exchange on ARF6. It is known to interact with Ptdlns (3,4,5) P3 localized to the plasma membrane in vitro and may also be a Ptdlns (3,4,5) P3 receptor. Additionally, GRP1 may regulate protein sorting and membrane trafficking through interaction with the guanosine triphosphate ARF, and may control cell adhesion through interaction with integrins.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Cytohesin-3; ARF nucleotide-binding site opener 3; Protein ARNO3; General receptor of phosphoinositides 1; Grp1; PH; SEC7 and coiled-coil domain-containing protein 3; ARNO3; GRP1; PSCD3; CYTH3
Swiss Prot
O43739
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 371-420 of Human GRP1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
GRP1 (K400) polyclonal antibody detects endogenous levels of GRP1 protein.
Applications Notes
Western blot (WB) analysis of GRP1 (K400) pAb at 1:2000 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:H9C2 whole cell lysate (40ug) Lane3:A2780 whole cell lysate (40ug) Lane4:H1792 whole cell lysate (40ug) Lane5:A375 whole cell lysate (20ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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