Synphilin-1 (R846) polyclonal antibody

Product Specifications

Background

Synphilin-1 (α-synuclein interacting protein, SNCAIP) is a 919 amino acid protein that associates with α synuclein and promotes the formation of cytosolic inclusions in neuronal cells. The synuclein family members, including α-synuclein and β-synuclein, are predominantly expressed in the brain, where they influence synaptic regulation and neuronal plasticity. Synphilin-1 contains modular protein domains, such as ankyrin-like repeats and a coiled-coil domain.While both α-synuclein and synphilin-1 are coexpressed in Lewy bodies of patients with Parkinson’s disease, only mutations in the gene for α-synuclein have been determined to confer pathogenicity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Synphilin-1; Sph1; Alpha-synuclein-interacting protein; SNCAIP

Swiss Prot

Q9Y6H5

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 815-867 of Human Synphilin-1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Synphilin-1 (R846) polyclonal antibody detects endogenous levels of Synphilin-1 protein.

Applications Notes

Western blot (WB) analysis of Synphilin-1 (R846) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)