GCSc-γ (E315) polyclonal antibody

Product Specifications

Background

The GCLC gene consists of 16 exons and encodes the 636 amino acid protein γ-GCSc (γ-glutamylcysteine synthetase heavy subunit), also designated γ-Lglutamate- L-cysteine ligase catalytic subunit (GLCLC) . γ-GCSc is expressed in hemocytes, brain, liver and kidney. γ-GCSc associates with a regulatory or modifier subunit, γ-GCSm (γ-glutamylcysteine synthetase light subunit), to form a heterodimer, γ-GCS. γ-GCS is the first enzyme involved and the rate determining step in glutathione iosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of γ-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of γ-GCS.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Glutamate--cysteine ligase catalytic subunit; 6.3.2.2; GCS heavy chain; Gamma-ECS Gamma-glutamylcysteine synthetase; GLCL; GLCLC; GCLC

Swiss Prot

P48506

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 300-350 of Human GCSc-γ.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GCSc-γ (E315) polyclonal antibody detects endogenous levels of GCSc-γ protein.

Applications Notes

Western blot (WB) analysis of GCSc-γ (E315) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (40ug) Lane2:NIH-3T3 whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug)