SOD1 (L85) polyclonal antibody
Product Specifications
Background
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS) . SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3) . SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1; SOD-1
Swiss Prot
P00441
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 61-110 of Human SOD1.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 18 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
SOD-1 (L85) polyclonal antibody detects endogenous levels of SOD-1 protein.
Applications Notes
Western blot (WB) analysis of SOD1 (L85) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit
Available Sizes
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