GPR44 (D113) polyclonal antibody

Product Specifications

Background

G protein-coupled receptors (GPCRs), such as GPR44, are integral membrane proteins containing 7 putative transmembrane domains (TMs) . These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response. GPR44 has recently been found to belong to the prostanoid receptor family and named DP2, with this becoming the official IUPHAR nomenclature. GPR44 has recently been found when activated by elevated level of prostaglandin D2 (PGD2), it could inhibit hair growth. This suggests the PGD2-GPR44 pathway as a potential target for bald treatment. A particularly promising compound for blocking the PGD2-GPR44 pathway is a compound known as TM-30089, which exhibits a "functional insurmountability" of PGD2 expression.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD_antigen=CD294; CRTH2; DL1R; GPR44; PTGDR2; DP2; DP-2

Swiss Prot

Q9Y5Y4

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 80-127 of Human GPR44.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 49 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GPR44 (D113) polyclonal antibody detects endogenous levels of GPR44 protein.

Applications Notes

Western blot (WB) analysis of GPR44 (D113) pAb at 1:1000 dilution Lane1:SGC7901 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug)