AChRα3 (L139) polyclonal antibody

Product Specifications

Background

The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Neuronal acetylcholine receptor subunit alpha-3; NACHRA3; CHRNA3

Swiss Prot

P32297

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-180 of Human AChRα3.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AChRα3 (L139) polyclonal antibody detects endogenous levels of Neuronal acetylcholine receptor subunit α3 protein.

Applications Notes

Western blot (WB) analysis of AChRα3 (L139) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)