SDHA (R600) polyclonal antibody

Product Specifications

Background

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; Flavoprotein subunit of complex II; Fp; SDHA; SDH2; SDHF

Swiss Prot

P31040

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 560-610 of Human SDHA.

Conjugation

Unconjugated

Related Pathways

Warburg Effect, Insulin &Glucose Signaling, AMPK Signaling Pathway

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 72 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SDHA (R600) polyclonal antibody detects endogenous levels of SDHA protein.

Applications Notes

Western blot (WB) analysis of SDHA (R600) pAb at 1:2000 dilution Lane1:The Heart tissue lysate of Rat (20ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:HepG2 whole cell lysate (40ug)