PRPF19 polyclonal antibody
Product Specifications
Background
The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6) . Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5‘ splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor; PRPF19I; NMP200; PRP19; SNEV
Swiss Prot
Q9UMS4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human PRPF19.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 55 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PRPF19 polyclonal antibody detects endogenous levels of PRPF19 protein.
Applications Notes
Western blot (WB) analysis of PRPF19 polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Rat (20ug) Lane2:The Brain tissue lysate of Mouse (20ug) Lane3:MCF-7 whole cell lysate (10ug) Lane4:Hela whole cell lysate (10ug)
Host or Source
Rabbit
Available Sizes
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