Spindlin-1 (H139) polyclonal antibody
Product Specifications
Background
SPIN1 (spindlin 1), also known as SPIN or ovarian cancer-related protein (OCR), is a 262 amino acid nuclear protein suggested to play a role in regulation of the cell-cycle during the transition from gamete to embryo. A member of the SPIN/STSY family, SPIN1 localizes to interphase nucleus and mitotic chromosomes, and is modified by phosphorylation in a cell-cycle-dependent fashion. A meiotic spindle-binding protein, SPIN1 overexpression has been shown to cause defects in mitotic spindles, thereby resulting in chromosome instability and potential tumorigenesis.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
OCR; Ovarian cancer-related protein; SPIN; Spin1; SPIN1; Spindlin 1
Swiss Prot
Q9Y657
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 110-156 of Human Spindlin-1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 30 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Spindlin-1 (H139) polyclonal antibody detects endogenous levels of Spindlin-1 protein.
Applications Notes
Western blot (WB) analysis of Spindlin-1 (H139) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:The Ovarian tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug)
Host or Source
Rabbit
Available Sizes
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