HLX1 (Q338) polyclonal antibody

Product Specifications

Background

HLX1 (homeobox protein HB24) is a 488 amino acid protein encoded by the human gene HLX1. HLX1 belongs to the H2.0 homeobox family and contains one homeobox DNA-binding domain. HLX1 is considered a diverged human homeodomain-containing protein known to be expressed in hematopoietic progenitors and activated lymphocytes. HLX1 is a transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. It is also involved in embryogenesis and hematopoiesis. Overexpression of HLX1 will cause a marked increase in activated T cells/thymocytes and interleukin-2 receptor expression. Overexpression will also impair the normal development of CD4+ T cells and will prevent the thymus from undergoing normal involution.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

H2.0 like homeobox 1 Drosophila; H2.0-like homeobox protein; HLX; HLX; Homeobox protein HB24; Homeobox protein HLX1

Swiss Prot

Q14774

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 303-360 of Human HLX1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HLX1 (Q338) polyclonal antibody detects endogenous levels of HLX1 protein.

Applications Notes

Western blot (WB) analysis of HLX1 (Q338) polyclonal antibody at 1:1000 dilution Lane1:JURKAT whole cell lysate (37ug) Lane2:THP-1 whole cell lysate (37ug) Lane3:The peripheral blood cell lysate of Rat (36ug) Lane4:The peripheral blood cell lysate of Mouse (36ug)