PKAα/β cat (K17) polyclonal antibody

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA) . Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

CAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACA; PKACA; PKA Ca; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB; PKACB; PKA Cb

Regulation of Actin Dynamics, Regulation of Microtubule Dynamics, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of PKAα/β cat (K17) polyclonal antibody at 1:500 dilution Lane1:The brain tissue lysate of Rat (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:MCF-7 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)