ETAR (N427) polyclonal antibody
Product Specifications
Background
Endothelin receptor A (ETAR), also known as EDNRA, ET1 receptor, ETA, EDN1 and ET-AR, is a member of the guanine-binding regulatory protein-coupled receptor family. ETAR binds endothelins and has the highest affinity for its ligand, ET1, as compared to the ETBR receptor. Both ET receptors, ETAR and ETBR, are activated by ET1, which results in inhibition of active lens sodiumpotassium transport. Activation of the ET receptors also causes an increase in cytoplasmic calcium concentration in cultured lens epithelial cells. In addition, ETAR induces arachidonic acid accumulation. ETAR has seven hydrophobic transmembrane domains and is expressed in aorta, lung, atrium, kidney, placenta and prostate. Specifically, placental vascular smooth muscle cells (PVSMCs) exclusively express ETAR.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Endothelin-1 receptor; Endothelin A receptor; ET-A; ETA-R; hET-AR; EDNRA; ETA; ETRA
Swiss Prot
P25101
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 402-452 of Human ETAR.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ETAR (N427) polyclonal antibody detects endogenous levels of ETAR protein.
Applications Notes
Western blot (WB) analysis of ETAR (N427) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:SGC7901 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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