Cadherin-8 (A519) polyclonal antibody

Product Specifications

Background

The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. Cadherin-8 expression has been seen in mouse thymus tissue as well as in specific subdivisions of the developing central nervous system. It plays a potential role in brain morphology.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cadherin 8; CADH8; Cadherin8

Swiss Prot

P55286

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 500-550 of Human Cadherin-8.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 88 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Cadherin-8 (A519) polyclonal antibody detects endogenous levels of Cadherin-8 protein.

Applications Notes

Western blot (WB) analysis of Cadherin-8 (A519) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:Mouse liver tissue lysate Lane3:Rat liver tissue lysate