Histone H2B (K24) polyclonal antibody
Product Specifications
Background
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ
Swiss Prot
Q93079
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Histone H2B.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 18 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Histone H2B (K24) polyclonal antibody detects endogenous levels of Histone H2B type 1-H protein.
Applications Notes
Western blot (WB) analysis of Histone H2B polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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