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Histone H2B (K24) polyclonal antibody

Product Specifications

Background

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B H3 and H4 and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H1 is required for the condensation of nucleosome chains into higher order structures. Phosporylation of Histone H1 is thought be involved in this process, although the exact nature of this role has yet to be elucidated. Evidence suggests that Histone H1 is a part of a general repressor mechanism for stable repression of transcription, but it can also activate transcription of specific genes.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Histone H2B type 1-H; Histone H2B.j; H2B/j; HIST1H2BH; H2BFJ

Swiss Prot

Q93079

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human Histone H2B.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Histone H2B (K24) polyclonal antibody detects endogenous levels of Histone H2B type 1-H protein.

Applications Notes

Western blot (WB) analysis of Histone H2B polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate

Host or Source

Rabbit

Available Sizes

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