SRp20 (F151) polyclonal antibody

Product Specifications

Background

The majority of SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 are continuously shuttled between the nucleus and the cytoplasm and contribute to mRNA transport. The activity of SR proteins in regulated splicing is antagonized by members of the hnRNP A/B family of proteins, which induce drastic shifts in the selection of splicing sites. An additional SR-associated protein, p32, tightly associates with SR factors and preferentially inhibits ASF/SF2 functioning as both a splicing enhancer and splicing repressor protein by preventing the stable interaction of ASF/SF2 and RNA.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/arginine-rich splicing factor 3; SFRS3; SRP20; SRSF3

Swiss Prot

P84103

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-170 of Human SRp20.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SRp20 (F151) polyclonal antibody detects endogenous levels of SRp20 protein.

Applications Notes

Western blot (WB) analysis of SRp20 (F151) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:Jurkat whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:BV2 whole cell lysate (40ug)