MGMT (C5) polyclonal antibody
Product Specifications
Background
MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53. Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
MGMT; EC 2.1.1.63; Methylated-DNA--protein-cysteine methyltransferase
Swiss Prot
P16455
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human MGMT.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MGMT (C5) polyclonal antibody detects endogenous levels of MGMT protein.
Applications Notes
Western blot (WB) analysis of MGMT (C5) pAb at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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