Dio-1 (S186) polyclonal antibody
Product Specifications
Background
Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFκB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Death-inducer obliterator 1; DIO-1; hDido1; Death-associated transcription factor 1; DATF-1; DIDO1; C20orf158; DATF1; KIAA0333
Swiss Prot
Q9BTC0
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 154-198 of Human Dio-1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 244 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Dio-1 (S186) polyclonal antibody detects endogenous levels of Dio-1 protein.
Applications Notes
Western blot (WB) analysis of Dio-1 (S186) pAb at 1:500 dilution Lane1:PC3 whole cell lysate (20ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC472062-500 1x 500 µL
TLE1 (Synovial Sarcoma Marker) (TLE1/2062), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
