Bcl-w (G154) polyclonal antibody

Product Specifications

Background

Using a PCR-based strategy, an additional protein with life-promoting activity, designated Bcl-w, has been identified. The protein is highly conserved between mouse and human and is encoded by a gene located near the TCRα gene on chromosome 14. Bcl-w is expressed in myeloid cell lines but not in T and B lymphocytes, and can be found in a wide range of tissues. An alternative splicing event in exon 4 results in two transcripts. The first, Bcl-w, encodes a protein of 193 amino acids, and the second, Bcl-w/rox, encodes a protein 333 amino acids in length. The “rox” portion of Bcl-w/rox shows a striking 66% amino acid sequence identity with the Drosophila rox2 protein; however, the Bcl-w/rox transcript may be expressed at very low levels.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Bcl-2-like protein 2; Bcl2-L-2; Apoptosis regulator Bcl-W; BCL2L2; BCLW; KIAA0271

Swiss Prot

Q92843

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 120-165 of Human Bcl-w.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 21 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Bcl-w (G154) polyclonal antibody detects endogenous levels of Bcl-w protein.

Applications Notes

Western blot (WB) analysis of Bcl-w (G154) pAb at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:SP2/0 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug)