RhoBTB1/2 (T85) polyclonal antibody

Product Specifications

Background

RHOBTB1 (Rho-related BTB domain-containing protein 1) and RHOBTB3 (Rho-related BTB domain-containing protein 3) each contain two BTB (POZ) domains and belong to the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. While both RHOBTB1 and RHOBTB3 are expressed ubiquitously, RHOBTB1 is found at high levels in placenta, stomach, testis, kidney and skeletal muscle, whereas RHOBTB3 is found at high levels in neural and cardiac tissues. RHOBTB1 is thought to play a role in GTPase-mediated signaling and may participate in organization of the Actin filament system. Additionally, RHOBTB1 expression is decreased in head and neck carcinomas, suggesting a possible role for RHOBTB1 as a tumor suppressor.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Rho-related BTB domain-containing protein 2; Deleted in breast cancer 2 gene protein; p83; RHOBTB2; DBC2; KIAA0717; Rho-related BTB domain-containing protein 1; RHOBTB1; KIAA0740

Swiss Prot

Q9BYZ6/O94844

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 52-98 of Human RhoBTB1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 83 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RhoBTB1/2 (T85) polyclonal antibody detects endogenous levels of RhoBTB1/2 protein.

Applications Notes

Western blot (WB) analysis of RhoBTB1/2 (T85) pAb at 1:1000 dilution Lane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)