TAF II p100 (G411) polyclonal antibody

Product Specifications

Background

TFIID is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide andother TATA-binding protein (TBP) -associated factors (TAFs) . Although native TFIID can mediate both activator-independent (basal) and activator-dependent transcription in reconstituted systems, TBP can mediate only basal transcription. TAF II p100 (TBP-associated factor II100), also known as TAF5 or TAFII100, is the third largest subunit of human TFIID. It contains six WD40 repeats at the C-terminus and has an N-terminus capable of forming a flexible dimer. TAF II p100 plays an important role in forming the scaffold that is crucial for the assembly of the TFIID complex. TAF II p100 may also be involved in the stabilization of TAF interactions.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transcription initiation factor TFIID subunit 5; Transcription initiation factor TFIID 100 kDa subunit; TAF (II) 100; TAFII-100; TAFII100; TAF5; TAF2D

Swiss Prot

Q15542

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 380-432 of Human TAF II p100.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TAF II p100 (G411) polyclonal antibody detects endogenous levels of TAF II p100 protein.

Applications Notes

Western blot (WB) analysis of TAF II p100 (G411) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:A2780 whole cell lysate (40ug) Lane3:The Lung tissue lysate of Rat (20ug)