UDG (H221) polyclonal antibody

Product Specifications

Background

The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Uracil-DNA glycosylase; UDG; UNG; DGU; UNG1; UNG15

Swiss Prot

P13051

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 200-240 of Human UDG.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

UDG (H221) polyclonal antibody detects endogenous levels of UDG protein.

Applications Notes

Western blot (WB) analysis of UDG (H221) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)