UDG (H221) polyclonal antibody
Product Specifications
Background
The human UNG-gene encodes nuclear (UNG2) and mitochondrial (UNG1) forms of uracil-DNA glycosylase using differentially regulated promoters, PA and PB, and alternative splicing to produce two proteins with unique N-terminal sorting sequences. Uracil in DNA may result from deamination of cytosine which can give rise to transition mutations. Uracil-DNA glycosylase is the DNA repair enzyme responsible for the removal of uracil from DNA. This is probably a biologically important function in the prevention of mutagenesis resulting from cytosine deamination. UNG2 removes misincorporated dUMP residues.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Uracil-DNA glycosylase; UDG; UNG; DGU; UNG1; UNG15
Swiss Prot
P13051
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 200-240 of Human UDG.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 35 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
UDG (H221) polyclonal antibody detects endogenous levels of UDG protein.
Applications Notes
Western blot (WB) analysis of UDG (H221) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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