ErbB2/HER2 (K676) polyclonal antibody
Product Specifications
Background
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a glycoprotein, undergoes transactivation upon hetero-dimerization with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; ERBB2; HER2; MLN19; NEU; NGL
Swiss Prot
P04626
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 641-690 of Human ErbB2/HER2.
Conjugation
Unconjugated
Related Pathways
ErbB HER Signaling
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 138,185 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ErbB2/HER2 (K676) polyclonal antibody detects endogenous levels of ErbB2/HER2 protein.
Applications Notes
Western blot (WB) analysis of ErbB2/HER2 (K676) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:Beas-2B whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:A375 whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (20ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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