PFKFB2 (Y14) polyclonal antibody
Product Specifications
Background
PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
PFK-2 car; PFK-2 cardiac; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase; PFKFB2
Swiss Prot
O60825
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human PFKFB2.
Conjugation
Unconjugated
Related Pathways
Warburg Effect, Glutamine Metabolism
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 58 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PFKFB2 (Y14) polyclonal antibody detects endogenous levels of PFKFB2 protein.
Applications Notes
Western blot (WB) analysis of PFKFB2 (Y14) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:U-87MG whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
