ARHGEF2 (P881) polyclonal antibody

Product Specifications

Background

GEFs catalyze the release of GDP from inactive Ras proteins to ena-ble GTP binding. Lfc (also designated Rho/Rac guanine nucleotide exchange factor 2, lymphoid blast crisis-like 1, ARHGEF2, GEF-H1, KIAA0651 or LFP40) is an 894 amino acid GEF for Rho A, a member of the Ras family that regulates the Actin cytoskeleton during the formation of stress fibers. Lfc directly interacts with Cingulin, a junctional adapter that inhibits Rho A, thus preventing phorbol-12-myristate-13-acetate-induced contraction and apoptosis. Mutations in the p53 gene transcriptionally activate Lfc, thereby increasing tumor cell proliferation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Rho guanine nucleotide exchange factor 2; Guanine nucleotide exchange factor H1; GEF-H1; Microtubule-regulated Rho-GEF; Proliferating cell nucleolar antigen p40; ARHGEF2; KIAA0651; LFP40

Swiss Prot

Q92974

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 850-897 of Human ARHGEF2.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Regulation of Microtubule Dynamics

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ARHGEF2 (P881) polyclonal antibody detects endogenous levels of ARHGEF2 protein.

Applications Notes

Western blot (WB) analysis of ARHGEF2 (P881) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate (40ug) Lane2:AML-12 whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:HuT78 whole cell lysate (20ug)