NF-YC (W85) polyclonal antibody

The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively.

Nuclear transcription factor Y subunit gamma; CAAT box DNA-binding protein subunit C; Nuclear transcription factor Y subunit C; NF-YC; Transactivator HSM-1/2; NFYC

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of NF-YC (W85) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate