Rad51C (E185) polyclonal antibody
Product Specifications
Background
The process of homologous recombination is a major DNA repair pathway that operates on DNA double-strand breaks to promote error-free repair. Human Rad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. In addition to Rad51 protein, five paralogs have been identified in vertebrates, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3. Rad51C binds single- and double-stranded DNA and preferentially binds 3'-end-tailed double-stranded DNA. Rad51C is expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; RAD51C; RAD51L2
Swiss Prot
O43502
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 161-210 of Human Rad51C.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 50 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Rad51C (E185) polyclonal antibody detects endogenous levels of Rad51C protein.
Applications Notes
Western blot (WB) analysis of Rad51C (E185) pAb at 1:500 dilution Lane1:H9C2 whole cell lysate (40ug) Lane2:The Testis tissue lysate of Mouse (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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