MSH3 (A80) polyclonal antibody

Product Specifications

Background

DNA mismatch repair (MMR) is essential for maintaining the integrity of the genome during replication. This process is highly conserved across bacterial and eukaryotic systems, as many of the genes expressed in bacteria are closely related to the yeast and mammalian homologs. In bacteria two proteins, MutS and MutL, form homodimeric complexes that are responsible for recognizing and facilitating MMR. Human homologs of these proteins include MSH2 and MSH3 (MutS homolog 2 and 3), and the corresponding human homologs of MutL are MLH1, PMS1, PMS2 and MLH3. MSH2 and MSH3 form heterodimers that cooperatively mediate MMR. MLH3 preferentially dimerizes with MLH1 to repair DNA mismatches and restore the stability to the genome.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

DNA mismatch repair protein Msh3; hMSH3; Divergent upstream protein; DUP; Mismatch repair protein 1; MRP1; MSH3; DUC1; DUG

Swiss Prot

P20585

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 50-100 of Human MSH3.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 127 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MSH3 (A80) polyclonal antibody detects endogenous levels of MSH3 protein.

Applications Notes

Western blot (WB) analysis of MSH3 (A80) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:H9C2 whole cell lysate (20ug) Lane4:CT26 whole cell lysate (40ug)