MLH1 (S467) polyclonal antibody

Product Specifications

Background

MLH1 is an integral part of the protein complex responsible for mismatch repair that is expressed in lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid and gall bladder and is methylated in several ovarian tumors. Loss of MLH1 protein expression is associated with a mutated phenotype, microsatellite instability and a predisposition to cancer. In hereditary nonpolyposis colorectal cancer (HNPCC), an autosomal dominant inherited cancer syndrome that signifies a high risk of colorectal and various other types of cancer, the MLH1 gene exhibits a pathogenic mutation. Certain cancer cell lines, including leukemia CCRF-CEM, colon HCT 116 and KM12, and ovarian cancers SK-OV-3 and IGROV-1, show complete deficiency of MLH1, while MLH1 is expressed in 60% of melanomas, 70% of noninvasive squamous cell carcinomas and 30% of invasive squamous cell carcinomas.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

DNA mismatch repair protein Mlh1; MutL protein homolog 1; COCA2

Swiss Prot

P40692

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 450-500 of Human MLH1.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 85 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

MLH1 (S467) polyclonal antibody detects endogenous levels of MLH1 protein.

Applications Notes

Western blot (WB) analysis of MLH1 (S467) pAb at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:A549 whole cell lysate (40ug) Lane5:MCF-7 whole cell lysate (40ug)