Elongin A3 (E403) polyclonal antibody

Product Specifications

Background

The elongin (SIII) complex is composed of a transcriptionally active A subunit and two small regulatory subunits, B and C. This complex stimulates the transcription elongation rate of RNA polyermase II by suppressing time spent at template-encoded arresting sites along DNA. Elongin A3, also known as RNA polymerase II transcription factor SIII subunit A3 and Transcription elongation factor B polypeptide 3C, is a 546 amino acid nuclear protein that functions as a general transcription elongation factor that increases RNA polymerase II transcription elongation and forms a stable complex with Elongin BC. In contrast to Elongin A, transcriptional activity of Elongin A3 is not stimulated by Elongin BC. Elongin A3 contains one TFIIS N-terminal domain and is ubiquitously expressed in human tissue.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

TCEB3C; Elongin-A3; Elongin A3; ElonginA3; EloA3; TCEB3L2

Swiss Prot

Q8NG57

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 370-422 of Human Elongin A3.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Elongin A3 (E403) polyclonal antibody detects endogenous levels of Elongin A3 protein.

Applications Notes

Western blot (WB) analysis of Elongin A3 (E403) polyclonal antibody at 1:500 dilution Lane1:Jurkat cell lysate Lane2:Mouse kidney tissue lysate Lane3:Rat kidney tissue lysate