CARKL (A53) polyclonal antibody
Product Specifications
Background
CARKL, also known as SHPK (sedoheptulokinase), is a 478 amino acid protein that localizes to the cytoplasm and belongs to the FGGY family of protein kinases. Expressed at high levels in kidney, pancreas and liver and at lower levels in heart, placenta, brain and lung, CARKL functions at an optimal pH of 8.5 and catalyzes the ATP-dependent phosphorylation of sedoheptulose to yield sedoheptulose 7-phosphate, an intermediate in the pentose phosphate pathway. Once phosphorylated, sedoheptulose is unable to exit the cell via the cell membrane, resulting in the containment of sedoheptulose 7-phosphate within the cell. Defects in the gene encoding CARKL are associated with cystinosis, an autosomal recessive genetic disorder of the renal tubules that is characterized by excessive urination and low blood levels of phosphates and potassium.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95; AKAP8; AKAP95
Swiss Prot
Q9UHJ6
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 333-393 of Human AKAP 95.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 51 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
CARKL pAb detects endogenous levels of CARKL protein.
Applications Notes
Western blot (WB) analysis of CARKL (A53) pAb at 1:500 dilution Lane1:The Kidney tissue lysate of Mouse (10ug) Lane2:The Kidney tissue lysate of Rat (10ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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