ARNT2 (M50) polyclonal antibody

Product Specifications

Background

AhR, ARNT1, ARNT2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Aryl hydrocarbon receptor nuclear translocator 2; ARNT protein 2; Class E basic helix-loop-helix protein 1; bHLHe1; ARNT 2; BHLHE1; KIAA0307

Swiss Prot

Q9HBZ2

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 20-69 of Human ARNT2.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 79 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ARNT2 (M50) polyclonal antibody detects endogenous levels of ARNT2 protein.

Applications Notes

Western blot (WB) analysis of Arnt2 (M50) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug)