ARNT2 (M50) polyclonal antibody
Product Specifications
Background
AhR, ARNT1, ARNT2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common “PAS” motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Aryl hydrocarbon receptor nuclear translocator 2; ARNT protein 2; Class E basic helix-loop-helix protein 1; bHLHe1; ARNT 2; BHLHE1; KIAA0307
Swiss Prot
Q9HBZ2
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 20-69 of Human ARNT2.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 79 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
ARNT2 (M50) polyclonal antibody detects endogenous levels of ARNT2 protein.
Applications Notes
Western blot (WB) analysis of Arnt2 (M50) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:H9C2 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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