PHKB (E689) polyclonal antibody

Product Specifications

Background

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Phosphorylase b kinase regulatory subunit beta; Phosphorylase kinase subunit beta; PHKB

Swiss Prot

Q93100

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 659-719 of Human PHKB.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PHKB (E689) polyclonal antibody detects endogenous levels of PHKB protein.

Applications Notes

Western blot (WB) analysis of PHKB (E689) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (30ug)