COPS5 / JAB1 (K191) polyclonal antibody

Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA) -induced expression of responsive genes through the TPA-response element (TRE) . The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. The coactivator of Jun, designated JAB1 (for Junactivation

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of COPS5/JAB1 (K191) pAb at 1:1000 dilution Lane1:A549 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:CT26 whole cell lysate (40ug)