Mob1A/B (D127) polyclonal antibody

Product Specifications

Background

MOB1 was first identified in yeast as a protein that binds to Mps with essential roles in the completion of mitosis and the maintenance of ploidy. Its Drosophila and mammalian homologs, Mats and MOB1, respectively, are involved in the Hippo signaling tumor suppressor pathway, which plays a critical role in organ size regulation and which has been implicated in cancer development. There are two MOB1 proteins in humans, MOB1α and MOB1β, that are encoded by two different genes but which have greater than 95% amino acid sequence identity. Both forms bind to members of the nuclear Dbf2-related (NDR) kinases, such as LATS1/2 and NDR1/2, thereby stimulating kinase activity. This binding is promoted by the phosphorylation of MOB1 at several threonine residues by MST1 and/or MST2.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

MOB kinase activator 1B; Mob1 homolog 1A; Mob1A; Mob1B; Mps one binder kinase activator-like 1A; MOB1B; MOB4A; MOBKL1A

Swiss Prot

Q7L9L4

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 97-157 of Human Mob1A/B.

Conjugation

Unconjugated

Related Pathways

Hippo Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Mob1A/B (D127) polyclonal antibody detects endogenous levels of Mob1A/B protein.

Applications Notes

Western blot (WB) analysis of Mob1A/B (D127) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:H1792 whole cell lysate (20ug) Lane3:HCT116 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug) Lane5:PMVEC whole cell lysate (40ug)