GK/GK3 (H39) polyclonal antibody

Product Specifications

Background

GK1 is a 559 amino acid mitochondrial peripheral membrane protein that belongs to the FGGY kinase family and is a key enzyme involved in the regulation of glycerol uptake and metabolism. GK shows high expression in kidney, testis and liver and exists as three isoforms, which are produced as a result of alternative splicing events.Glycerol kinase 3 is a key enzyme in the regulation of glycerol uptake and metabolism. Glycerol kinase 3 (GKP3, GK3) could be the product of a pseudogene.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Putative glycerol kinase 3; GK 3; Glycerokinase 3; ATP:glycerol 3-phosphotransferase 3; Glycerol kinase, testis specific 1; GK3P; GKP3; GKTB; Glycerol kinase; Glycerokinase; ATP:glycerol 3-phosphotransferase; GK; GK1

Swiss Prot

P32189/Q14409

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 3-60 of Human GK.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 61 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GK/GK3 (H39) polyclonal antibody detects endogenous levels of GK and GK3 protein.

Applications Notes

Western blot (WB) analysis of GK/GK3 (H39) pAb at 1:2000 dilution Lane1:SP2/0 whole cell lysate (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:HepG2 whole cell lysate (20ug)