EKLF (W318) polyclonal antibody

Product Specifications

Background

The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF. EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arrest.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Krueppel-like factor 1; Erythroid krueppel-like transcription factor; EKLF; KLF1; EKLF

Swiss Prot

Q13351

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 211-311 of human EKLF (NP_006554.1) .

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

EKLF (W318) polyclonal antibody detects endogenous levels of EKLF protein.

Applications Notes

Western blot (WB) analysis of EKLF polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (30ug) Lane2:Jurkat whole cell lysate (30ug) Lane3:THP-1 whole cell lysate (30ug) Lane4:SHSY5Y whole cell lysate (30ug)