FoxC1/2 (K138) polyclonal antibody
Product Specifications
Background
The forkhead transcription factor genes FOXC1 (Mf1) and FOXC2 (Mfh1) interact with the Notch signaling pathway and are required for the prepatterning of anterior and posterior domains in the presumptive somites through a putative Notch/Delta/Mesp regulatory loop. The genes have similar, dose-dependent functions, and compensate for each other in the early development of the heart, blood vessels and somites. Both FOXC1 and FOXC2 are expressed in the mesenchyme from which the ocular drainage structures derive. FOXC1 and FOXC2 also interact in kidney and heart development. Mutations in the FOXC1 gene result in Axenfeld-Rieger malformations of the anterior segment of the eye and lead to an increased susceptibility of glaucoma, including juvenile glaucoma. Functional regions in FOXC1 are required for nuclear localization and transcriptional regulation. Specifically, two regions in the FOXC1 forkhead domain, one rich in basic amino acid residues, and a second, highly conserved among all FOX proteins, are necessary for nuclear localization of the FOXC1 protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Forkhead box protein C1; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; FREAC-3; FOXC1; FKHL7; FREAC3; Forkhead box protein C2; Forkhead-related protein FKHL14; Mesenchyme fork head protein 1; MFH-1 protein; Transcription factor FKH-14; FOXC2; FKHL14; MFH1
Swiss Prot
Q12948/Q99958
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 100-150 of Human FoxC1.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
FoxC1/2 (K138) polyclonal antibody detects endogenous levels of FoxC1 and FoxC2 protein.
Applications Notes
Western blot (WB) analysis of FoxC1/2 (K138) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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