DMPK (R30) polyclonal antibody

Product Specifications

Background

Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3’-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP) /SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM) .

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myotonin-protein kinase; MT-PK; DM-kinase; DMK; DM1 protein kinase; DMPK; Myotonic dystrophy protein kinase; DMPK; DM1PK; MDPK

Swiss Prot

Q09013

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminus of Human DMPK.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DMPK (R30) polyclonal antibody detects endogenous levels of DMPK protein.

Applications Notes

Western blot (WB) analysis of DMPK (R30) pAb at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (20ug)