DMPK (R30) polyclonal antibody
Product Specifications
Background
Myotonic dystrophy protein kinase (DMPK) is a ulti-domain protein kinase found in muscle that is activated in response to G protein second messengers and proteolysis. DMPK is implicated in myotonic muscular dystrophy (DM), an autosomal dominant-inherited disorder that predominately affects skeletal and cardiac muscle and causes defects in cardiac conduction. DM arises through expansion of CTG repeats in the 3’-UTR of the DMPK gene. Mutant DMPK transcripts with an extended region of CUG repeats are retained in the nucleus. These transcripts also influence the expression of the DM locus-associated homeodomain protein (DMAHP) /SIX5, to mediate in part the DM phenotype. Other substrates for DMPK include myogenin, Ltype calcium channels, and Phospholemman (PLM) .
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Myotonin-protein kinase; MT-PK; DM-kinase; DMK; DM1 protein kinase; DMPK; Myotonic dystrophy protein kinase; DMPK; DM1PK; MDPK
Swiss Prot
Q09013
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human DMPK.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 70 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
DMPK (R30) polyclonal antibody detects endogenous levels of DMPK protein.
Applications Notes
Western blot (WB) analysis of DMPK (R30) pAb at 1:500 dilution Lane1:CT26 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (20ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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