CtBP2 (P432) polyclonal antibody

Product Specifications

Background

CtBP1 and the related protein CtBP2 are characterized as C-terminal binding proteins of adenovirus E1A, and they preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation.CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of deltaEF1, a cellular zinc finger-homeodomainprotein, and thereby enhances deltaEF1-induced transcriptional silencing. In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

C-terminal-binding protein 2; CtBP2; CTBP2

Swiss Prot

P56545

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 400-450 of Human CtBP2.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~48.0 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CtBP2 (P432) polyclonal antibody detects endogenous levels of CtBP2 protein.

Applications Notes

Western blot (WB) analysis of CtBP2 (P432) polyclonal antibody at 1:500 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug)