HnRNP H (R192) polyclonal antibody

Product Specifications

Background

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, including hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly (rG) regions on RNA.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Heterogeneous nuclear ribonucleoprotein H2; hnRNP H2; FTP-3; Heterogeneous nuclear ribonucleoprotein H'; hnRNP H'; HNRNPH2; FTP3; HNRPH2

Swiss Prot

P55795

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 180-230 of Human hnRNP H.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HnRNP H (R192) polyclonal antibody detects endogenous levels of hnRNP H protein.

Applications Notes

Western blot (WB) analysis of hnRNP H (R192) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:C6 whole cell lysate (40ug) Lane4:CT26 whole cell lysate (40ug)