Casein Kinase Iε (P301) polyclonal antibody

Product Specifications

Background

Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, γ, ε and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The α catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Casein kinase I isoform epsilon; CKI-epsilon; CKIe; CSNK1E; CKIE; CK-IE

Swiss Prot

P49674

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 250-350 of Human Casein Kinase Iε.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 47 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Casein Kinase Iε (P301) polyclonal antibody detects endogenous levels of Casein Kinase Iε protein.

Applications Notes

Western blot (WB) analysis of Casein Kinase Iε (P301) pAb at 1:500 dilution Lane1:3T3-L1 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)