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Myf-5 (A88) polyclonal antibody

Product Specifications

Background

Myf5 is a member of the myogenic basic helix-loop-helix family of transcription factors. These activate the muscle differentiation program. Myf5 also induces fibroblasts to differentiate into myoblasts. It is a probable sequence specific DNA-binding protein.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2; MYF5; BHLHC2

Swiss Prot

P13349

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 60-100 of Human Myf-5.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 28 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Myf-5 (A88) polyclonal antibody detects endogenous levels of Myf-5 protein.

Applications Notes

Western blot (WB) analysis of Myf-5 (A88) pAb at 1:1000 dilution Lane1:PC3 whole cell lysate (20ug) Lane2:U-87MG whole cell lysate (20ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:BV2 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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