HnRNP A1 (E85) polyclonal antibody
Product Specifications
Background
HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
HnRNP core protein A1-like protein; RP11-78J21.1
Swiss Prot
P09651
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 51-100 of Human hnRNP A1.
Conjugation
Unconjugated
Applications
WB IHC IF
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 33 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HnRNP A1 (E85) polyclonal antibody detects endogenous levels of hnRNP A1 protein.
Applications Notes
Western blot (WB) analysis of hnRNP A1 (E85) pAb at 1:2000 dilution Lane1:L02 whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:HEK293T whole cell lysate (10ug) Lane4:The Lung tissue lysate of Mouse (40ug) Lane5:The Lung tissue lysate of Rat (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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