HnRNP A1 (E85) polyclonal antibody

HnRNP A1 belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre mRNAs in the nucleus and appear to influence pre mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP A1 has two repeats of quasi RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re imported.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of hnRNP A1 (E85) pAb at 1:2000 dilution Lane1:L02 whole cell lysate (10ug) Lane2:MCF-7 whole cell lysate (10ug) Lane3:HEK293T whole cell lysate (10ug) Lane4:The Lung tissue lysate of Mouse (40ug) Lane5:The Lung tissue lysate of Rat (40ug)