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Prefoldin 1 (E35) polyclonal antibody

Product Specifications

Background

PFDN1 is a heterohexameric chaperone protein which assists in the correct folding of other proteins. It binds specifically to cytosolic chaperonin and transfers target proteins. PFDN1 may function by selectively targeting nascent actin and tubulin chains pending their transfer to cytosolic chaperonin for final folding and/or assembly. It promotes folding in an environment in which there are many competing pathways for non native proteins.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

PFDN1; PDF; PFD1; Prefoldin subunit 1

Swiss Prot

O60925

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 11-60 of Human Prefoldin 1.

Conjugation

Unconjugated

Applications

WB

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 14 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Prefoldin 1 (E35) polyclonal antibody detects endogenous levels of Prefoldin 1 protein.

Applications Notes

Western blot (WB) analysis of Prefoldin 1 (E35) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:U-87MG whole cell lysate (40ug) Lane5:HEK293T whole cell lysate (40ug) Lane6:H9C2 whole cell lysate (40ug) Lane7:CT-26 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

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