RNF15 (E193) polyclonal antibody

Product Specifications

Background

Tripartite motif-containing protein 38 (TRIM38), also known as RING finger protein 15 (RNF15) or zinc finger protein RoRet, is a 465 amino acid member of the TRIM family, also known as the RING-B-box coiled-coil (RBCC) family. Members of the RBCC family have an N-terminal RING finger, followed by one or two zinc-binding domains (B-box domains), a leucine coiled-coil region and a variable C-terminal domain. Found in all eukaryotes, members of the RBCC family typically function within a larger protein complex and possess ubiquitin-protein isopeptide ligase activity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RING finger protein 15; Zinc finger protein RoRet; TRIM38; MGC8946. RNF15. RORET

Swiss Prot

O00635

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 171-220 of Human RNF15.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500-1:2000 IHC: 1:100-1:300

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 58 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RNF15 (E193) polyclonal antibody detects endogenous levels of RNF15 protein.

Applications Notes

Western blot (WB) analysis of RNF15 (E193) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:3T3-L1 whole cell lysate (40ug)