AHA-1 (T183) polyclonal antibody

Product Specifications

Background

AHA-1 stimulates the inherent ATPase activity of yeast and human HSP 90 and interacts with the cytoplasmic tail of vesticular stomatitis virus glycoprotein. AHA-1 regulates HSP 90 by influencing the conformational state of the “ATP lid” and consequent N-terminal dimerization. It is crucial for cell viability under non-optimal growth conditions when HSP 90 levels are limiting. AHA-1 is a cytosolic protein and may transiently interact with the endoplasmic reticulum. It can have an affect on one step in the endoplasmic to Golgi trafficking. AHA-1 is expressed in numerous tissues, including brain, heart, skeletal muscle and kidney, and at lower levels in liver and placenta. It is induced by heat shock and treatment with the HSP 90 inhibitor 17-demethoxygeldanamycin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

AHSA1; AHA1; C14ORF3; Activator of 90 kDa heat shock protein ATPase homolog 1; HSPC322

Swiss Prot

O95433

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to amino acids 151-200 of Human AHA-1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 38 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AHA-1 (T183) polyclonal antibody detects endogenous levels of AHA-1 protein.

Applications Notes

Western blot (WB) analysis of AHA-1 (T183) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:786-O whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:U87-MG whole cell lysate (40ug)